Changes

RIPA Buffer

32 bytes added, 15:22, 7 August 2013
RIPA Buffer (for 10mL lysis buffer)
! || Final Concentration || per 10 mL || Stock || Location
|-
|Tris pH7.4 || 50mM || 500uL || 1M || Chemical Shelf
|-
|Na Deoxycholate || 0.25% || 250uL || 10% || Chemical Shelf
|-
| NP-40 || 1% || 1mL || 10% || TingtingChemical Shelf
|-
|NaCl || 150mM || 375uL || 4M || Shelf
|EDTA || 1mM || 20uL || 0.5M || Shelf
|-
|NaVO3 || 100uM || 10uL || 100mM || Biochemical Stocks (-20)Chemical Shelf
|-
|NaF || 5mM || 100uL || 500mM || Chemical Shelf
|-
|NaPPi || 10 mM || 1 mL || 100mM || Chemical Shelf
|-
| Protease Inhibitors || || 1 mini tablet || || 4C
|}
[[ Category:Buffer ]]
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