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Created page with 'Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see link). For each well in a 12 well plate lyse in 1 ml. rotate 0.5 ml lysat...'
Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see link).
For each well in a 12 well plate lyse in 1 ml.
rotate 0.5 ml lysate with 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz).
For each well in a 12 well plate lyse in 1 ml.
rotate 0.5 ml lysate with 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz).