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Immunoprecipitation

30 bytes added, 17:12, 4 April 2012
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==Protocol==* Lyse cells in lysis buffer, such as RIPA buffer with protease inhibitors and phosphatase inhibitors (see link[[RIPA Buffer]]).* For each well in a 12 well plate lyse in 1 ml.* rotate 0.5 ml lysate with 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz).

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