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GoTaq PCR Genotyping

37 bytes added, 14:01, 16 September 2010
edited primer concentration
==Materials==
*GoTaq Master Mix
*Primer Mix (100uL Primersfrom 100uM Primer Stocks): Add 10uL of forward and reverse primer, 20uL total, into 1mL 980uL of dH20to make a 10uM solution.
*Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 14uL Nuclease-Free Water, and 1uL of template
*Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr.