9
edits
Changes
no edit summary
==Materials(100uM)==
*GoTaq Master Mix
*Primer Mix(100uL Primers): Add 10uL of forward and reverse primer, 20uL total, into 1mL of dH20.
*Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 14uL Nuclease-Free Water, and 1uL of template
*Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr.
