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PCR Analysis of Tail DNA

4 bytes removed, 17:59, 5 June 2009
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==Protocol==
 #Use the following Volumes per Reaction: #Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer)  #Forward Primer: .4ul #Reverse Primer: .4ul #dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer) #Sterile water: 13.6 uL  #Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer)  #Template: 1 uL  
Run PCR Program (approx 2 hours).
Use Cycler 1 on 6th Floor
 *Login: Sergey, Just press enter to Login *Under Genotype folder, pick Ingles program for Ingles genotyping *Under Genotype folder, pick regpcr program for PLT genotyping
Make sure to press enter 2x once to confirm Tubes and second time to start PCR
 
see [[Preparing an Agarose Gel]] for details on preparing a DNA gel