162
edits
Changes
→Crosslinking, Lysis and Shearing of DNA
11. Resuspend pellet to 1 ml with RIPA Buffer in a '''15 mL falcon tube''' (Do not vortex the tubes and try to avoid bubbles. Bubbles will cause popping and loss of samples during sonication).
''Note: Remember to take a portion of this resuspended pellet prior to lysing to compare unshared to sheared DNA.''
12. Using the Sonics VibraCell Sonicator, sonicate each 1.0 ml ChIP sample on ice, in a cold room, at Power Output 5 watts 6 times for 30 seconds each