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2. Add formaldehyde to a final concentration of 1% directly to the media of adherent cells growing on tissue culture plates, swirl gently, and
incubate at room temperature for 10 minutes.
* If using 10cm dishes add 270ul 250ul of 3740% formaldehyde
3. Stop the cross-linking reaction by adding glycine to a final concentration of 0.125M and swirl gently to mix.