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→Reverse cross-linking and recover ChIP DNA
==== Reverse cross-linking and recover ChIP DNA ====
# Incubate bead pellet from step 2.f above in a 65°C water bath for '''1 hour''', shake or vortex every 15 minutes to elute the immuno-bound chromatin from the beads.
# Spin at 14,000 rpm in a microfuge at room temperature for 3 minutes.
# Collect the supernatant, which contains the ChIP’d DNA. The tubes can be placed on the magnet to facilitate supernatant recovery.