User contributions

1 April 2014

• BDNF PCR

  Created page with "'''BDNF Primers''' ---- * oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common) * oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse) * oIMR01694 --- GGG AAC TTC CTG ACT AG..."

  14:13

• Main Page

  Added BNDF

  14:03

  +13

19 February 2014

• Rapamycin Injections

  no edit summary

  15:03

  -1

1 November 2013

• Paraffin Embedding of Tissue Samples

  Created page with " == Protocol == #After harvesting tissue from animal, place into pathology cassette and fix in 4% formaldehyde, 4% paraformaldehyde or 4% formalin O/N #After fixation, begin d..."

  15:09

• Main Page

  ‎Tissue Preparation

  14:50

  +42

13 September 2013

• Fly Food Protocol

  ‎Cooking Insturctions

  13:56

3 September 2013

• Fly Food Protocol

  ‎Cooking Insturctions

  17:37

  +7

• Fly Food Protocol

  Created page with " == Quantities == '''Summer''' #900mL - Corn Syrup #202.4g - Yeast #117.2g - Soy Flour #856g - Corn Meal #67.6g - Agar #15L - Water #65mL - Propionic Acid..."

  17:36

• Main Page

  ‎Fly Stocks

  16:29

  +23

7 August 2013

• RIPA Buffer

  ‎RIPA Buffer (for 10mL lysis buffer)

  15:23

  +17

• RIPA Buffer

  ‎RIPA Buffer (for 10mL lysis buffer)

  15:22

  +32

• RIPA Buffer

  ‎RIPA Buffer (for 10mL lysis buffer)

  15:21

6 August 2013

• Maintenance of 18C Fly Stocks

  ‎=Maintenance

  16:09

  +1

• Maintenance of 18C Fly Stocks

  Created page with "==Location== Drosophila stock vials can be found in the 18C cooler of the Reiter Laboratory ==Maintenance= *Drosophila stocks are to be flipped every 3 weeks *When flipping t..."

  16:09

• Main Page

  ‎Fly Stocks

  16:04

• Maintanence of 18C Fly Stocks

  Created page with "==Location== Drosophila stocks can be found in the 18C cooler of the Reiter Lab ==Maintenance== *Drosophila stocks are to be "flipped" every 3 weeks *Always flip the oldest o..."

  16:02

• Main Page

  ‎Mouse/Fly Protocols

  15:55

  +51

• Main Page

  ‎Mouse Protocols

  15:54

  +4

1 August 2013

• Triglyceride Assay from Drosophila (German Method)

  ‎Protocol

  14:29

  +102

31 July 2013

• Triglyceride Assay from Cells and Tissues

  ‎Protocol

  21:26

  +1,305

• Triglyceride Assay from Drosophila (German Method)

  Created page with "==Protocol== #Use 5 female or 8 Male flies for Assay #Homogenize flies (3 min @ 40Hz) in 1mL of .05% Tween (from Tween-20 stock) #Heat samples in 70C waterbath for 5 minutes #..."

  21:12

• Triglyceride Assay from Cells and Tissues

  ‎Protocol: Creation of Protocol

  21:11

  -1,406

• Main Page

  ‎Protein Purification

  m

  21:05

  +56

26 July 2013

• Preparation of Protein Lysates from Mouse Tissues

  ‎Protocol

  m

  21:27

  +13

• Bradford Assay

  ‎Protocol: Added Protein Lysate

  m

  21:23

  +266

• PCR Analysis of Tail DNA

  ‎Protocol

  13:55

  +39

25 July 2013

• PCR Analysis of Tail DNA

  ‎Protocol: Master Mix

  m

  15:00

  +247

11 July 2013

• Western Blotting

  ‎Protocol: LiCor Addition

  m

  19:39

  +133

• Transfer Buffer

  ‎10X Transfer Buffer: Changed from 10L total to 4L total

  m

  19:34

  +2

• Western Blotting

  ‎Protocol: Changed wash times

  m

  19:33

  -6

28 June 2013

• Triglyceride Assay from Cells and Tissues

  ‎Protocol: Volume of butanol mixture

  m

  17:10

26 June 2013

• Preparation of Tail Samples (for Genotyping)

  ‎Protocol: Proteinase K

  m

  17:18

  +31

19 June 2013

• Triglyceride Assay from Cells and Tissues

  Deletion of unnecessary information (1/10 Glycerol Dilution)

  m

  15:29

  -55

• Triglyceride Assay from Cells and Tissues

  updated volumes and lysis procedure

  15:24

  -466

• Bridges Lab Protocols

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