User contributions
10 May 2018
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+48
Chromatin Immunoprecipitation
Prior to starting this section:
+6
Chromatin Immunoprecipitation
Reverse Crosslinks of Protein/DNA Complexes to Free DNA
+18
10 January 2018
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+2
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+122
9 January 2018
Chromatin Immunoprecipitation
Prior to starting this section:
-8
ChIP Elution Buffer
no edit summary
ChIP Elution Buffer
no edit summary
+313
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+6
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+2
Chromatin Immunoprecipitation
Before You Start
+57
Dilution Buffer
no edit summary
+337
ChIP Elution Buffer
Created page with "10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water."
Dilution Buffer
Created page with "0.01% SDS, 1.1% Triton X- 100, 1.2mM EDTA, 16.7mM Tris-HCl, pH 8.1, 167mM NaCl."
Chromatin Immunoprecipitation
Buffers and Solutions Needed
+22
Chromatin Immunoprecipitation
Buffers and Solutions Needed
+139
Chromatin Immunoprecipitation
Buffers and Solutions Needed
-79
TE Buffer
Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | Tris|| 10mM|| 150uL || 1M || Solutions Shelf |- | EDTA || 1mM || 30uL || 500mM..."
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
High Salt Immune Complex Wash Buffer
Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | SDS || 0.1% || 150uL || 10% || Solutions Shelf |- | Triton X-100 || 1% || 1.5mL..."
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
Low Salt Immune Complex Wash Buffer
no edit summary
+6
LiCl Immune Complex Wash Buffer
Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | NP40 || 1% || 1.5mL || 1..."
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+4
Low Salt Immune Complex Wash Buffer
no edit summary
-21
Low Salt Immune Complex Wash Buffer
Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | Example || Example || Ex..."
Chromatin Immunoprecipitation
Prior to starting this section:
-11
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+3
Chromatin Immunoprecipitation
Reverse Crosslinks of Protein/DNA Complexes to Free DNA
+6
Chromatin Immunoprecipitation
Prior to starting this section:
-1
Chromatin Immunoprecipitation
Immunoprecipitation
+16
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
+6
Chromatin Immunoprecipitation
Crosslinking, Lysis and Shearing of DNA
+19
Chromatin Immunoprecipitation
Immunoprecipitation
+58
Chromatin Immunoprecipitation
no edit summary
-4
Chromatin Immunoprecipitation
Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads
-32
Chromatin Immunoprecipitation
Immunoprecipitation
+3,234
5 January 2018
15 December 2017
Preparation of Protein Lysates from Cells
no edit summary
-161
Preparation of Protein Lysates from Cells
Created page with "==Materials== *RIPA Buffer (see RIPA) or other Lysis buffer. Add protease inhibitors. *Cells (fresh or frozen) ==Protocol== #Cool centrifuge to 4C #If cells..."
24 October 2017
Submitting Plasmids for Sequencing
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Submitting Plasmids for Sequencing
no edit summary
+159
19 October 2017
Cloning CRISPR-Cas Plasmids
no edit summary
+114
Submitting Plasmids for Sequencing
no edit summary
+32
Submitting Plasmids for Sequencing
no edit summary
+752
Submitting Plasmids for Sequencing
Created page with "*Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul *These should be submitted in 1.5mL tubes *Go to https://client-seqcore.brcf.med.um..."