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• Real Time PCR From Cell Culture
  *SyberGreen PCR Master Mix Applied Biosystems ===RT-PCR Reaction===
  3 KB (423 words) - 00:42, 29 March 2011
• PCR Amplification of DNA
  *DreamTaq Green PCR Master mix - contains dNTPs, polymerase, salts, and buffer with loading dye ==PCR Program==
  1 KB (162 words) - 16:57, 8 June 2020
• PCR Analysis of Tail DNA
  Run "specfic" PCR Program for gene of interest (approx 2 hours). *[[PCR Amplification of DNA]]
  773 bytes (111 words) - 17:00, 8 June 2020
• Colony PCR
  #Prepare PCR: ===Run PCR Program Colony PCR:===
  690 bytes (108 words) - 15:43, 21 October 2009
• PCR Amplification of DNA (KOD Polymerase)
  *Run PCR Program (approx 3.5 to 4 hours). Normally use touchdown PCR ('''TD-KOD''') as follows: *Purify PCR product if necessary using Qiagen kit (Add 5x PB)
  1 KB (179 words) - 21:24, 3 November 2009
• GoTaq PCR Genotyping
  *Prepare gel 30 minutes before PCR is finished. *Add this mixture to each PCR tube.
  1 KB (134 words) - 14:50, 6 December 2010
• Cre PCR
  ==PCR Program== [[ Category:PCR]]
  301 bytes (43 words) - 18:42, 25 April 2018
• Quantifying Relative Expression from qRT-PCR
  2 KB (250 words) - 14:57, 2 December 2013
• PCR Amplification with Platinum Taq High Fidelity
  * Platinum PCR SuperMix High Fidelity (Life Technologies cat# 12532-016) * The PCR program should be
  745 bytes (105 words) - 13:43, 17 July 2015
• BDNF PCR
  491 bytes (80 words) - 14:13, 1 April 2014
• RT-PCR primer design for ChIP
  # PCR product size should be set to 70-150bp. [[ Category: PCR ]]
  1 KB (224 words) - 14:24, 21 August 2016

Page text matches

• Real Time PCR From Cell Culture
  *SyberGreen PCR Master Mix Applied Biosystems ===RT-PCR Reaction===
  3 KB (423 words) - 00:42, 29 March 2011
• Main Page
  *[[PCR Amplification of DNA]] *[[Real Time PCR From Cell Culture]]
  4 KB (484 words) - 12:10, 15 August 2016
• Mutagenesis
  #Run PCR Program (Mutagenesis 8/15/20 minute, depending on template)
  1 KB (208 words) - 15:15, 13 January 2010
• Restriction Enzyme Based Cloning
  #If insert is PCR, run PCR then PCR purify (Qiagen kit). Elute in 30 uL EB
  1 KB (227 words) - 14:40, 7 May 2009
• TOPO Cloning
  #PCR desired insert using Pfu polymerase. Add 1 uL of Taq and mix. Incubate 10 #If PCR product is a single band, no need to purify further. If several bands, gel
  611 bytes (109 words) - 00:45, 2 May 2009
• PCR Amplification of DNA
  *DreamTaq Green PCR Master mix - contains dNTPs, polymerase, salts, and buffer with loading dye ==PCR Program==
  1 KB (162 words) - 16:57, 8 June 2020
• PCR Analysis of Tail DNA
  Run "specfic" PCR Program for gene of interest (approx 2 hours). *[[PCR Amplification of DNA]]
  773 bytes (111 words) - 17:00, 8 June 2020
• QPCR - Lin Lab
  ==PCR Program:== Protocol written for 96-well Real-time PCR machine in Jiandie Lin lab on 10/4/2006.
  3 KB (562 words) - 12:13, 28 May 2009
• Designing and Preparing dsRNA
  *set up a 250 uL PCR reaction **15 uL MgCl (this can be adjusted to optimize PCR conditions)
  2 KB (290 words) - 14:31, 1 October 2009
• Colony PCR
  #Prepare PCR: ===Run PCR Program Colony PCR:===
  690 bytes (108 words) - 15:43, 21 October 2009
• PCR Amplification of DNA (KOD Polymerase)
  *Run PCR Program (approx 3.5 to 4 hours). Normally use touchdown PCR ('''TD-KOD''') as follows: *Purify PCR product if necessary using Qiagen kit (Add 5x PB)
  1 KB (179 words) - 21:24, 3 November 2009
• GoTaq PCR Genotyping
  *Prepare gel 30 minutes before PCR is finished. *Add this mixture to each PCR tube.
  1 KB (134 words) - 14:50, 6 December 2010
• Preparing an Adenoviral shRNA Clone
  ...ligo + 48 uL annealing buffer (100 mM NaCl and 50 mM HEPES pH 7.4.) into a PCR tube.
  2 KB (353 words) - 19:47, 13 December 2010
• Genotyping Program
  |[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || aTSC, mTSC, Placenta TSC || Two bands if heter |[[Cre Genotyping|Cre]] || [[Cre PCR ]] || Between 340-400kDa || aTSC, mTSC, Adipo-Raptor, Adipo-GR-KO, Placenta
  2 KB (297 words) - 14:32, 23 July 2021
• Preparation of Tail Samples (for Genotyping)
  ...Proteinase K and mouse tail in an eppendorf tube or in a well of a 96 well PCR plate (Proteinase K stock = 10mg/mL in ddH2O)
  1 KB (184 words) - 15:20, 21 May 2018
• Rapid Isolation of Genomic DNA from Yeast
  [[Category:PCR]]
  1 KB (197 words) - 16:32, 21 February 2012
• Verifying Kan Deletion Mutants from Genomic DNA
  [[Category:PCR]] ...[[Rapid Isolation of Genomic DNA from Yeast]]. Use 1µl of the DNA in your PCR reaction.
  1 KB (248 words) - 16:39, 21 February 2012
• CAP Genotyping
  [[Category: PCR ]] *Get sequences from Xiao-ling, stocks in XL PCR Box
  281 bytes (35 words) - 17:26, 27 February 2012
• First Strand cDNA Synthesis (AB Kit)
  * Run the PCR Program '''AB Reaction Protocol'''. Takes ~2h:
  1 KB (154 words) - 15:16, 24 July 2019
• Vinexin Genotyping
  [[ Category:PCR]] ==PCR Program==
  2 KB (225 words) - 15:14, 6 August 2012

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