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Preparing an Agarose Gel

Revision as of 16:03, 5 May 2009 by Davebridges (Talk | contribs) (wrote page)

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Revision as of 16:03, 5 May 2009 by Davebridges (Talk | contribs) (wrote page)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Materials

  • Agarose
  • TAE Buffer (1X): Dilute from 100X stock
  • BioRad Gel Apparatus (Mold, Holder, Comb and Gel Rig)

Prepare Gel (BioRad Gel Rig)

  1. Place plastic mold in holder and turn handle to tighten
  2. Put 15 or 8 well comb in mold as appropriate
  3. Weight out 0.3g agarose and add 30 mL of TAE
  4. Microwave 40s until dissolved
  5. Add 1 uL ethidium bromide
  6. Pour into mold
  7. Place gel in cold room if in a hurry
  8. After loading, run gel at 100V and dispose in ethidium bromid waste
Last modified on 24 August 2016, at 19:22