PCR Analysis of Tail DNA

see Genotyping Details for strain specific details

Use the following Volumes per Reaction:

Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer)

Forward Primer: .4ul

Reverse Primer: .4ul

dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer)

Sterile water: 13.6 uL

Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer)

Template: 1 uL

see Preparing an Agarose Gel for details on preparing a DNA gel