Genotyping Program
| Allele | Program | Fragment Size(s) | Notes | |||||
|---|---|---|---|---|---|---|---|---|
| TSC-floxed | Cre PCR | KO- 368bp, WT- 295bp | Two bands if heterozygote | |||||
| Cre | Cre PCR | Only present if Transgene Positive (cannot detect homozygosity at transgene) | ||||||
| Vac14 | Cre PCR | Two bands if heterozygote | ||||||
| TC10b | TC10b | KO - 500bp, WT-1kb | Two bands if heterozygote | ob/ob Genotyping | ob/ob | KO - 123bp, WT-104kb, control 191 bp | Two reactions, see ob/ob Genotyping | |
| MTM1 | MTM1 | Mutant-575bp, WT-714bp | Separate Reactions for WT and KO | |||||
| CAP | CAP | Two bands if heterozygote, upper band for KO | ||||||
| Vinexin | Vinexin | |||||||
| GR KO | nr3c1 | KO- 300bp, WT- 247bp | Two bands if heterozygote | |||||
| mc4r-Cone | mc4r | KO- 540, WT- 400 | Two bands if heterozygote (run primers separately |