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PCR Analysis of Tail DNA

Revision as of 18:36, 17 February 2016 by Lmcallan (Talk | contribs) (→‎Protocol)

Revision as of 18:36, 17 February 2016 by Lmcallan (Talk | contribs) (→‎Protocol)

see Genotyping Details for strain specific details

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Contents

Materials

  1. Dream Taq Green master mix
  2. Specific Primers
  3. DNA
  4. ddH2O

Protocol

Use the following Volumes per 25ul Reaction:

Per sample 1X

  1. Dream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer)
  2. Primer Mix: 5ul
  3. Sterile ddH2O: 7.5ul
  • Template: 1 uL


Run "specfic" PCR Program for gene of interest (approx 2 hours).

  • specific to each gene


see Preparing an Agarose Gel for details on preparing a DNA gel

Last edited 5 years ago by Reddj