Maternal Particulate Exposure Breeding

Ordering

• Order C57BL6 mice at 42 days of age from jaxmice.org
• Once the mice arrive, place mice on breeder's chow for seven days
• At 49 days of age, mate female and male mice.

Exposure

• On day 10 and 17 of gestation expose the mice to particulate or control (saline/cabosil).
  • Should be close to days 59 and 66 days of age.

Weaning

• Keep the mother's on breeder's chow through weaning.