Main Page

Cell and Tissue Culture

• Splitting Cells
• Differentiation of 3T3-L1 Cells
• Immunofluoresence
• 3T3-L1 Adipocyte Fractionation

Cloning and Molecular Biology

• Transformation of Bacteria
• Mutagenesis
• PCR Amplification of DNA
• Restriction Enzyme Based Cloning
  • Restriction Enzyme Based Cloning - Ordering Primers
• TOPO Cloning
• Preparing an Agarose Gel

Protein Analysis

• Bradford Assay
• Western Blotting
• Surface Plasmon Resonance - Protein Lipid Interactions

Protein Purification

• French Press
• Purification of GST Fusion Proteins

Transcriptional Analysis

• Real Time PCR From Cell Culture

Consult the User's Guide for information on using the wiki software.