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Revision as of 13:31, 13 May 2009 by
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Transcriptional Analysis
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Revision as of 13:31, 13 May 2009 by
Davebridges
(
Talk
|
contribs
)
(
→
Transcriptional Analysis
)
(
diff
)
← Older revision
|
Latest revision
(
diff
) |
Newer revision →
(
diff
)
Contents
Cell and Tissue Culture
Cloning and Molecular Biology
Protein Analysis
Protein Quantification
Protein Purification
Transcriptional Analysis
Mouse Protocols
Genotyping
Cell and Tissue Culture
Splitting Cells
Differentiation of 3T3-L1 Cells
Electroporation of 3T3-L1 Adipocytes
Immunofluoresence
3T3-L1 Adipocyte Fractionation
Preparing Cell Lysates
Harvesting RNA from Cells grown in monolayer
Cloning and Molecular Biology
Transformation of Bacteria
Mutagenesis
PCR Amplification of DNA
Restriction Enzyme Based Cloning
Restriction Enzyme Based Cloning - Ordering Primers
TOPO Cloning
Preparing an Agarose Gel
Protein Analysis
Western Blotting
Surface Plasmon Resonance - Protein Lipid Interactions
Protein Quantification
Bradford Assay
Quantification by Absorbance at 280nm
Protein Purification
French Press
Purification of GST Fusion Proteins
Determining Percent Purity
Transcriptional Analysis
Real Time PCR From Cell Culture
Harvesting RNA from Cells grown in monolayer
Mouse Protocols
Genotyping
Ear Tagging and Tail Clipping
DNA Preparation from Tail Clip
PCR Analysis of Tail DNA
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