High Fat Diet Treatment

To place mice on a high fat diet, here is the general protocol:

For C57BL/6J mice, order so that they are 9 weeks of age on the monday of delivery. Start the diet on the Wednesday (10 days later).

Diet Choices

For more information about diets see research diets

Food Souces and Notes about their Composition

Food	Vendor	Code	Caloric Content	Fat	Protein	Sucrose	Starch	Notes
Normal Chow Diet	Lab Diet	5L0D	3.02 kcal/g	5%	24%	3.7%	32%	Provided by ULAM
High Fat Diet	Research Diets	D12451	3.85 kcal/g	45%	20%	17%	7%	Pink
Control Diet (High Sucrose)	Research Diets	D12450B	3.85 kcal/g	10%	20%	35%	31%	Yellow
Control Diet (High Starch)	Research Diets	D12450H	3.85 kcal/g	10%	20%	17%	44%	Peach

Treatment Protocol

1. Start when mice are 10 weeks of age.
2. For High Fat Diet mice place in cages 4 mice/cage as early as possible. Can us 5 mice/cage for normal chow/control diets.
3. Re-cage (and ear tag mice if necessary) mice and replace food with ~400g food in the container. Record this weight.
4. This food should last 2 weeks.
5. Weigh mice at ~10 AM either weekly or bi-weekly and re-weigh food to determine food intake. Top up to 400g.
6. Generally treatment lasts 12 weeks.