Genotyping Program

Allele	Program	Fragment Size(s)	Notes
TSC-floxed	Cre PCR	KO- 368bp, WT- 295bp	Two bands if heterozygote
Cre	Cre PCR		Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14	Cre PCR		Two bands if heterozygote
TC10b	TC10b	KO - 500bp, WT-1kb	Two bands if heterozygote		ob/ob Genotyping	ob/ob	KO - 123bp, WT-104kb, control 191 bp	Two reactions, see ob/ob Genotyping
MTM1	MTM1	Mutant-575bp, WT-714bp	Separate Reactions for WT and KO
CAP	CAP		Two bands if heterozygote, upper band for KO
Vinexin	Vinexin
GR KO	nr3c1	KO- 300bp, WT- 247bp	Two bands if heterozygote
mc4r-Cone	mc4r	KO- 540, WT- 400	Two bands if heterozygote (run primers separately