Difference between revisions of "Preparing an Agarose Gel"

Revision as of 16:03, 5 May 2009

Materials

• Agarose
• TAE Buffer (1X): Dilute from 100X stock
• BioRad Gel Apparatus (Mold, Holder, Comb and Gel Rig)

Prepare Gel (BioRad Gel Rig)

1. Place plastic mold in holder and turn handle to tighten
2. Put 15 or 8 well comb in mold as appropriate
3. Weight out 0.3g agarose and add 30 mL of TAE
4. Microwave 40s until dissolved
5. Add 1 uL ethidium bromide
6. Pour into mold
7. Place gel in cold room if in a hurry
8. After loading, run gel at 100V and dispose in ethidium bromid waste