Purification of GST-HA-S6K: Difference between revisions
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updated dna amount in materials (was ok in protocol) |
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__NOTOC__ | __NOTOC__ | ||
==Materials== | ==Materials== | ||
*GST-HA-S6K1 plasmid. Prepare by [[Cesium Chloride Preparation of DNA]]. Need | *GST-HA-S6K1 plasmid. Prepare by [[Cesium Chloride Preparation of DNA]]. Need 500 ug per 10 plates of cells | ||
*293A Cells. | *293A Cells. | ||
*Glutathione-Sepharose | *Glutathione-Sepharose | ||