Glucose Uptake Assay: Difference between revisions
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#Add 400 uL cells to 5 mL aqueous scintillation fluid, vortex and count on program 3 | #Add 400 uL cells to 5 mL aqueous scintillation fluid, vortex and count on program 3 | ||
#Do a bradford assay on 20 uL of cells (use PBS as blank) | #Do a bradford assay on 20 uL of cells (use PBS as blank) | ||
==Analysis== | |||
*For excel fill in orange cells in this template found on Github [https://github.com/davebridges/biomolecule-scripts/blob/master/Excel%20Templates/2-DG%20Template.xlsx] | |||
*For sweave use this template found on Github [https://github.com/davebridges/biomolecule-scripts/blob/master/R/Sweave/2-dg%20analysis.Rnw] | |||
[[Category: Metabolic Measurements]] | [[Category: Metabolic Measurements]] | ||
[[Category: Glucose Uptake]] | [[Category: Glucose Uptake]] | ||
[[Category: Cell Culture]] | [[Category: Cell Culture]] | ||