Glucose Uptake Assay: Difference between revisions
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==Materials== | ==Materials== | ||
*2-Deoxyglucose (cold): Sigma D-6134. Dissolve 32.8mg/mL (200 mM) in KRBH | *2-Deoxyglucose (cold): Sigma D-6134. Dissolve 32.8mg/mL (200 mM) in [[KRBH Buffer]] | ||
*0.5% BSA in [[KRBH Buffer]] | *0.5% BSA in [[KRBH Buffer]] | ||
*Radioactive <sup>14</sup>C-2-deoxyglucose | *Radioactive <sup>14</sup>C-2-deoxyglucose | ||