PCR Analysis of Tail DNA: Difference between revisions
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#Use the following Volumes per Reaction: | #Use the following Volumes per Reaction: | ||
#Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer) | #Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer) | ||
#Forward Primer: .4ul | #Forward Primer: 0.4ul | ||
#Reverse Primer: .4ul | #Reverse Primer: 0.4ul | ||
#dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer) | #dNTPs: 0.4uL of 2 mM ("Molecular Biology Stuff" box in freezer) | ||
#Sterile water: 13.6 uL | #Sterile water: 13.6 uL | ||
#Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer) | #Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer) | ||