QPCR: Difference between revisions
→Real Time qPCR: updated catalog numbers |
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==Real Time qPCR== | ==Real Time qPCR== | ||
===Materials=== | ===Materials=== | ||
*cDNA | *cDNA: see [[First_Strand_cDNA_Synthesis_(AB_Kit)|First Strand cDNA Synthesis (AB Kit)]] for details | ||
*SyberGreen PCR Master Mix Applied Biosystems | *SyberGreen PCR Master Mix Applied Biosystems (ThermoFisher Catalog # 4367659; [https://www.thermofisher.com/order/catalog/product/4367659 Vendor Link]) | ||
*384 well qPCR plate | *384 well qPCR plate (ThermoFisher Catalog # 4309849) and covers (Catalog # 4360954) | ||
*Primers (Dilute to 0.4 uM mixture of fwd and rev. From 100 uM stocks this is 4uL Forward Primer, 4 uL Reverse Primer and 992 uL Water). | *Primers (Dilute to 0.4 uM mixture of fwd and rev. From 100 uM stocks this is 4uL Forward Primer, 4 uL Reverse Primer and 992 uL Water). This primer mix can be stored at -20 in a Working Primers box. Design primers according to [[Primer Design for qPCR]] | ||
===Plate Preparation=== | ===Plate Preparation=== | ||