Designing Clones Using Benchling: Difference between revisions
Created page with "First, go to [https://benchling.com] to create an account. The following protocol is a tutorial for designing clones using the Gibson method: #Click the "Create+" tab at the t..." |
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#When you are happy with everything, you can choose the "finalize" option (located under the 'assembly' tab). | #When you are happy with everything, you can choose the "finalize" option (located under the 'assembly' tab). | ||
#Benchling will automatically design primers for your assembly (they will be towards the beginning and end of the regions of your gene and to those connecting portions of the plasmid around the insert), you can find the forward and reverse primer sequences under the 'assembly' tab as well. They will show up when you click on them. From here you can just copy and paste the primer sequences into the IDT website to order. | #Benchling will automatically design primers for your assembly (they will be towards the beginning and end of the regions of your gene and to those connecting portions of the plasmid around the insert), you can find the forward and reverse primer sequences under the 'assembly' tab as well. They will show up when you click on them. From here you can just copy and paste the primer sequences into the IDT website to order. | ||
#There is an option to create a PDF document to save your assembly to your computer if you choose to do so, otherwise it will be available on the website. | |||