Chromatin Immunoprecipitation: Difference between revisions
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==== Reverse cross-linking and recover ChIP DNA ==== | ==== Reverse cross-linking and recover ChIP DNA ==== | ||
# Incubate | # Incubate beads from above in a 65°C water bath for '''1 hour''', shake or vortex every 15 minutes to elute the immuno-bound chromatin from the beads. | ||
# Spin at 14,000 rpm in a microfuge at room temperature for 3 minutes. | # Spin at 14,000 rpm in a microfuge at room temperature for 3 minutes. | ||
# Collect the supernatant, which contains the ChIP’d DNA. The tubes can be placed on the magnet to facilitate supernatant recovery. | # Collect the supernatant, which contains the ChIP’d DNA. The tubes can be placed on the magnet to facilitate supernatant recovery. | ||