Chromatin Immunoprecipitation: Difference between revisions
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''Note: This treatment breaks the cells while keeping the nuclei mostly intact.'' | ''Note: This treatment breaks the cells while keeping the nuclei mostly intact.'' | ||
10. Collect the crude nuclear prep by centrifuging at 2,000 rpm at | 10. Collect the crude nuclear prep by centrifuging at 2,000 rpm at 4°C for 5 minutes. | ||
11. Resuspend pellet to 1 ml with RIPA Buffer. | 11. Resuspend pellet to 1 ml with RIPA Buffer. | ||