Difference between revisions of "RIPA Buffer"

From Bridges Lab Protocols
Jump to: navigation, search
(RIPA Buffer (for 10mL lysis buffer))
(RIPA Buffer (for 10mL lysis buffer): added link to orthovanadate protocol)
 
Line 13: Line 13:
 
|EDTA || 1mM || 20uL || 0.5M ||Chemical Shelf
 
|EDTA || 1mM || 20uL || 0.5M ||Chemical Shelf
 
|-  
 
|-  
|NaVO3 || 100uM || 10uL || 100mM || Chemical Shelf
+
|NaVO3 (see [[Preparation_of_Orthovanadate_Stocks | preparation ]]) || 100uM || 10uL || 100mM || Chemical Shelf
 
|-  
 
|-  
 
|NaF || 5mM || 100uL || 500mM || Chemical Shelf
 
|NaF || 5mM || 100uL || 500mM || Chemical Shelf

Latest revision as of 20:57, 27 January 2016

RIPA Buffer (for 10mL lysis buffer)

Final Concentration per 10 mL Stock Location
Tris pH7.4 50mM 500uL 1M Chemical Shelf
Na Deoxycholate 0.25% 250uL 10% Chemical Shelf
NP-40 1% 1mL 10% Chemical Shelf
NaCl 150mM 375uL 4M Chemical Shelf
EDTA 1mM 20uL 0.5M Chemical Shelf
NaVO3 (see preparation ) 100uM 10uL 100mM Chemical Shelf
NaF 5mM 100uL 500mM Chemical Shelf
NaPPi 10 mM 1 mL 100mM Chemical Shelf
Protease Inhibitors 1 mini tablet 4C