Difference between revisions of "RIPA Buffer"

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(RIPA Buffer (for 10mL lysis buffer))
(RIPA Buffer (for 10mL lysis buffer))
Line 9: Line 9:
 
| NP-40 || 1% || 1mL || 10% || Chemical Shelf
 
| NP-40 || 1% || 1mL || 10% || Chemical Shelf
 
|-  
 
|-  
|NaCl || 150mM || 375uL || 4M || Shelf
+
|NaCl || 150mM || 375uL || 4M || Chemical Shelf
 
|-  
 
|-  
|EDTA || 1mM || 20uL || 0.5M || Shelf
+
|EDTA || 1mM || 20uL || 0.5M ||Chemical Shelf
 
|-  
 
|-  
 
|NaVO3 || 100uM || 10uL || 100mM || Chemical Shelf
 
|NaVO3 || 100uM || 10uL || 100mM || Chemical Shelf

Revision as of 15:23, 7 August 2013

RIPA Buffer (for 10mL lysis buffer)

Final Concentration per 10 mL Stock Location
Tris pH7.4 50mM 500uL 1M Chemical Shelf
Na Deoxycholate 0.25% 250uL 10% Chemical Shelf
NP-40 1% 1mL 10% Chemical Shelf
NaCl 150mM 375uL 4M Chemical Shelf
EDTA 1mM 20uL 0.5M Chemical Shelf
NaVO3 100uM 10uL 100mM Chemical Shelf
NaF 5mM 100uL 500mM Chemical Shelf
NaPPi 10 mM 1 mL 100mM Chemical Shelf
Protease Inhibitors 1 mini tablet 4C