Triglyceride Assay from Cells and Tissues: Difference between revisions
m Deletion of unnecessary information (1/10 Glycerol Dilution) |
m →Protocol: Volume of butanol mixture |
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| Line 18: | Line 18: | ||
# Dry in fume hood overnight (or until completely dry) | # Dry in fume hood overnight (or until completely dry) | ||
# If absorbance is going to be measured by cuvette, use non-bolded values. If you are using a plate reader use bolded values. | # If absorbance is going to be measured by cuvette, use non-bolded values. If you are using a plate reader use bolded values. | ||
# Add | # Add 500uL '''(50uL)''' of '''Butanol Mixture'''. See [[#Suggested Volumes | Suggested Volumes]] for your specific tissue | ||
# Measure triglyceride levels using Sigma Diagnostic Kit using 5 uL of sample: | # Measure triglyceride levels using Sigma Diagnostic Kit using 5 uL of sample: | ||
## Resuspend triglyceride and glycerol reagent with water if necessary. | ## Resuspend triglyceride and glycerol reagent with water if necessary. | ||