Genotyping Program: Difference between revisions

Line 2:

!| Allele || Program|| Fragment Size(s) || Notes

|-

|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || || Two bands if heterozygote

|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || Two bands if heterozygote

|-

|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || Only present if Transgene Positive (cannot detect homozygosity at transgene)

Line 9:

|-

|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote

|-

|

|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]

|-

|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-~~575~~, WT-~~714~~ || Separate Reactions for WT and KO

|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575bp, WT-714bp || Separate Reactions for WT and KO

|-

|[[CAP Genotyping|CAP]] || CAP || || Two bands if heterozygote, upper band for KO

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|[[Vinexin Genotyping|Vinexin]] || Vinexin || ||

|-

|[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- ~~300~~, WT- ~~247~~ || Two bands if heterozygote

|[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300bp, WT- 247bp || Two bands if heterozygote

|-

|[[mc4r-Cone Genotyping| mc4r-Cone]] || mc4r || KO- 540, WT- 400 || Two bands if heterozygote (run primers separately

|}

[[ Category:Genotyping ]]

[[ Category:Mouse Work]]

@@ Line 2: / Line 2: @@
 !| Allele || Program|| Fragment Size(s) || Notes
 |-
-|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] ||  || Two bands if heterozygote
+|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || Two bands if heterozygote
 |-
 |[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || Only present if Transgene Positive (cannot detect homozygosity at transgene)
@@ Line 9: / Line 9: @@
 |-
 |[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
-|-
+|
 |[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]
 |-
-|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575, WT-714  || Separate Reactions for WT and KO
+|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575bp, WT-714bp  || Separate Reactions for WT and KO
 |-
 |[[CAP Genotyping|CAP]] || CAP ||  || Two bands if heterozygote, upper band for KO
@@ Line 18: / Line 18: @@
 |[[Vinexin Genotyping|Vinexin]] || Vinexin || ||
 |-
-|[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300, WT- 247 || Two bands if heterozygote
+|[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300bp, WT- 247bp || Two bands if heterozygote
+|-
+|[[mc4r-Cone Genotyping| mc4r-Cone]] || mc4r || KO- 540, WT- 400 || Two bands if heterozygote (run primers separately
 |}
 [[ Category:Genotyping ]]
 [[ Category:Mouse Work]]