Dilution Buffer: Difference between revisions

Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/Dilution_Buffer"

@@ Line 1: / Line 1: @@
-.01% SDS, 1.1% Triton X- 100, 1.2mM EDTA, 16.7mM Tris-HCl, pH 8.1, 167mM NaCl.
+{| class="wikitable"
+|-
+! Chemical !! Final Concentration !! Per 50mL !! Stock !! Location
+|-
+| SDS || 0.01% || 50uL|| 10% || Solutions Shelf
+|-
+| Triton X-100 || 1.1% || 5.5mL || 10% || Solutions Shelf (borrowed from Seo lab to make stock)
+|-
+| EDTA || 1.2mM || 120uL || 500mM || Solutions Shelf
+|-
+| Tris (pH 8.1) || 16.7mM || 835uL || 1M || Solutions Shelf
+|-
+| NaCl || 167mM || 2.09mL || 4M || Solutions Shelf
+|}