Tamoxifen Injections for Inducible Knockouts: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
* Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil) | * Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil)-- must wear nitrile gloves when dealing with tamoxifen. | ||
* Inject 50 mg/kg on five consecutive days, at the same time each day. The time is important, otherwise they will overdose if you inject too close together. | * Inject 50 mg/kg on five consecutive days, at the same time each day. The time is important, otherwise they will overdose if you inject too close together. | ||
* To determine how much to inject weigh the mouse and inject 5 uL/g of mouse. This means a 25 g mouse will get an injection of 125 uL. | * To determine how much to inject weigh the mouse and inject 5 uL/g of mouse. This means a 25 g mouse will get an injection of 125 uL. | ||
* Give yourself plenty of time to fill needles because the corn oil takes a long time to aspirate--watch for bubbles. | * Give yourself plenty of time to fill needles because the corn oil takes a long time to aspirate--watch for bubbles. | ||
* Tamoxifen can be stored at room temperature for up to a week. | * Tamoxifen can be stored at room temperature for up to a week. | ||
* The initial weights can be used for the rest of the week when determining injection volume, unless there is reason to believe they will be changing drastically. | |||
* Notes about transferring mice: | * Notes about transferring mice: | ||