RT-PCR primer design for ChIP: Difference between revisions

← Older edit Newer edit →

@@ Line 1: / Line 1: @@
-# Locate the potential gene regions you believe your protein is bound to (for glucocorticoid-induced ChIP-seq peaks refer to [[Locating ChIP-seq peaks from ENCODE]]. Make sure this the genetic sequence is species appropriate.  If not, you can use the BLAT option from the UCSC website [https://genome.ucsc.edu/index.html] and choose the desired species from the drop-down menu.
+# Locate the potential gene regions you believe your protein is bound to (for ChIP-seq peaks refer to [[Locating ChIP-seq peaks from ENCODE]]. Make sure this the genetic sequence is species appropriate.  If not, you can use the BLAT option from the UCSC website [https://genome.ucsc.edu/index.html] and choose the desired species from the drop-down menu.
 # The genetic region entered for primer search should be around 400 bp.
 # Go to NCBI primer design [http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?ORGANISM=9606&INPUT_SEQUENCE=NM_001618.3]