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Glycogen Determination from Tissues

66 bytes added, 14:26, 8 June 2012
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## Add 700 uL Glucose Buffer Solution with Color Reagent to a plastic cuvette. (for microplate add 100ul)
## Add 1-5 uL glucose standard (500mg/dL) for standard curve (for microplate add 1-5 ul of glucose standard 200mg/dL diluted 1:5)
## Add 10 uL digested glycogen(for mice fasted more than 6 hours. for fed/short fast need less)
## Mix and incubate at 37C for 5 min
## Measure absorbance at 505 nm
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