22
edits
Changes
no edit summary
# Resuspend pellets in 200uL amyloglucosidase solution and incubate at 37C for 3h-O/N
# Quantify glucose using kit:
## Add 700 uL Glucose Buffer Solution with Color Reagent to a plastic cuvette.(for microplate add 100ul)## Add 1-5 uL glucose standard (500mg/dL) for standard curve(for microplate add 1-5 ul of glucose standard 200mg/dL diluted 1:5)
## Add 10 uL digested glycogen
## Mix and incubate at 37C for 5 min
