Adipocyte Cell Counting with ImageJ: Difference between revisions
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****Select "MRI_Adipocyte_Tools" from downloads to install. | ****Select "MRI_Adipocyte_Tools" from downloads to install. | ||
==Adipocyte Cell Counting== | ==Adipocyte Cell Counting== | ||
*With macro installed, click on segmentation: "s" | |||
**Set boundaries for sizing of cell | |||
***Use 40-40,000 for size typically. | |||
**Change thresholding method to Default. | |||
*Open image of interest. | |||
*Click on segmentation "s" | |||
*Select freehanding tool to outline any cells that were not counted | |||
**To add freehanded cell to ROI manager, click command + T | |||
*Once all cells have been outlined, and misnumbered/outlined cells have been removed, click on measure | |||
**Copy and save measurements to Excel spreadsheet | |||
===To save image of outlined cells=== | |||
*Make sure all cells are outlined. | |||
*Click on "Flatten" | |||
*Save flattened image to folder. | |||