PCR Analysis of Tail DNA: Difference between revisions
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see [[Genotyping | see [[Genotyping Program]] for strain specific details | ||
==Materials== | ==Materials== | ||
# Dream Taq Green master mix | |||
# Specific gene Primers (0.4um Working stock) | |||
# Tail digest DNA | |||
# ddH2O | |||
==Protocol== | ==Protocol== | ||
First, make 250ul of 0.4um working stock primers from 100um Primary Stocks. | |||
#248 ul ddH20 | |||
#1ul forward primer (100um) | |||
#1ul reverse primer (100um) | |||
Use the following Volumes per 25ul Reaction: | |||
Per sample (1X) | |||
#Dream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer) | |||
#0.4um Primer Mix: 5ul | |||
#Sterile ddH2O: 7.5ul | |||
*Template: 1 uL | |||
Run "specfic" PCR Program for gene of interest (approx 2 hours). | |||
*[[Genotyping Program]] | |||
*[[PCR Amplification of DNA]] | |||
see [[Preparing an Agarose Gel]] for details on preparing a DNA gel | see [[Preparing an Agarose Gel]] for details on preparing a DNA gel | ||
[[Category: Genotyping]] | |||
[[Category: Mouse Work]] | |||