Difference between revisions of "Preparing an Agarose Gel"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (wrote page) |
m (→Prepare Gel (BioRad Gel Rig)) |
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*Agarose | *Agarose | ||
*TAE Buffer (1X): Dilute from 100X stock | *TAE Buffer (1X): Dilute from 100X stock | ||
| − | * | + | *Thermo Apparatus (Mold, Holder, Comb and Gel Rig) |
==Prepare Gel (BioRad Gel Rig)== | ==Prepare Gel (BioRad Gel Rig)== | ||
| − | #Place plastic mold in holder | + | #Place plastic mold securely in holder |
| − | #Put | + | #Put well comb in mold |
| − | #Weight out | + | #Weight out 1.0g agarose and add 100 mL of TAE (1% solution) then return TAE to shelf |
| − | #Microwave | + | #Microwave 90s until dissolved |
| − | #Add | + | #Add 2 uL Gel Red. Swirl to mix and eliminate sediments |
#Pour into mold | #Pour into mold | ||
#Place gel in cold room if in a hurry | #Place gel in cold room if in a hurry | ||
| − | #After loading, run gel at 100V | + | #After loading, run gel at 100V |
Latest revision as of 19:22, 24 August 2016
Materials
- Agarose
- TAE Buffer (1X): Dilute from 100X stock
- Thermo Apparatus (Mold, Holder, Comb and Gel Rig)
Prepare Gel (BioRad Gel Rig)
- Place plastic mold securely in holder
- Put well comb in mold
- Weight out 1.0g agarose and add 100 mL of TAE (1% solution) then return TAE to shelf
- Microwave 90s until dissolved
- Add 2 uL Gel Red. Swirl to mix and eliminate sediments
- Pour into mold
- Place gel in cold room if in a hurry
- After loading, run gel at 100V
