Difference between revisions of "ChIP Elution Buffer"
From Bridges Lab Protocols
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− | OR you can make this way but the stocks for this are not made up, it ends up being the | + | OR you can make this way but the stocks for this are not made up, it ends up being the same final concentrations |
10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water. | 10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water. |
Latest revision as of 22:09, 9 January 2018
Chemical | Final Concentration | Per 5mL | Stock | Location |
---|---|---|---|---|
SDS | 1% | 500uL | 10% | Solutions Shelf |
NaHCO3 | 0.1M | 42g | Powder | Chemical Shelf |
OR you can make this way but the stocks for this are not made up, it ends up being the same final concentrations 10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water.