Difference between revisions of "Dilution Buffer"
From Bridges Lab Protocols
(Created page with "0.01% SDS, 1.1% Triton X- 100, 1.2mM EDTA, 16.7mM Tris-HCl, pH 8.1, 167mM NaCl.") |
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| − | 0.01% | + | {| class="wikitable" |
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| + | ! Chemical !! Final Concentration !! Per 50mL !! Stock !! Location | ||
| + | |- | ||
| + | | SDS || 0.01% || 50uL|| 10% || Solutions Shelf | ||
| + | |- | ||
| + | | Triton X-100 || 1.1% || 5.5mL || 10% || Solutions Shelf (borrowed from Seo lab to make stock) | ||
| + | |- | ||
| + | | EDTA || 1.2mM || 120uL || 500mM || Solutions Shelf | ||
| + | |- | ||
| + | | Tris (pH 8.1) || 16.7mM || 835uL || 1M || Solutions Shelf | ||
| + | |- | ||
| + | | NaCl || 167mM || 2.09mL || 4M || Solutions Shelf | ||
| + | |} | ||
Latest revision as of 21:29, 9 January 2018
| Chemical | Final Concentration | Per 50mL | Stock | Location |
|---|---|---|---|---|
| SDS | 0.01% | 50uL | 10% | Solutions Shelf |
| Triton X-100 | 1.1% | 5.5mL | 10% | Solutions Shelf (borrowed from Seo lab to make stock) |
| EDTA | 1.2mM | 120uL | 500mM | Solutions Shelf |
| Tris (pH 8.1) | 16.7mM | 835uL | 1M | Solutions Shelf |
| NaCl | 167mM | 2.09mL | 4M | Solutions Shelf |
