Submitting Plasmids for Sequencing: Difference between revisions

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*Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul

*These should be submitted in 1.5mL tubes

*Go to https://client-seqcore.brcf.med.umich.edu/login.cgi?exp=1

**PI login: Bridges

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**Do not rush

*Save and repeat with your other samples

*Go to ... for locations and drop off times

*Once you have finished adding your samples ID codes for each sample will be generated and the last four digits of this ID are what you need to use for the tubes you are submitting, not the sample names you made

**Samples should be submitted in 1.5mL tubes

**Make sure to write these IDs very clearly

**A submission ID will also be generated and you need to know this when you drop your samples off so they can find your sequence request in their system

**Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul and should be labeled with the generated sample ID

*In addition to your samples you will need to provide an aliquot of the primer

**Volume is 10ul/sample you submit

**Should be labeled as the range of sample IDs generated and P1 (example: xxxx-xxxxP1), if you have more than one primer write xxxx-xxxxP2 and so on

*Go to https://seqcore.brcf.med.umich.edu/sample-dropoff-locations for locations and drop off times

*They will send you an email when the project has been completed. You can download the .ab1 files here: https://client-seqcore.brcf.med.umich.edu/ftpdata.cgi

@@ Line 1: / Line 1: @@
-*Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul
-*These should be submitted in 1.5mL tubes
 *Go to https://client-seqcore.brcf.med.umich.edu/login.cgi?exp=1
 **PI login: Bridges
@@ Line 18: / Line 16: @@
 **Do not rush
 *Save and repeat with your other samples
-*Go to ... for locations and drop off times
+*Once you have finished adding your samples ID codes for each sample will be generated and the last four digits of this ID are what you need to use for the tubes you are submitting, not the sample names you made
+**Samples should be submitted in 1.5mL tubes
+**Make sure to write these IDs very clearly
+**A submission ID will also be generated and you need to know this when you drop your samples off so they can find your sequence request in their system
+**Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul and should be labeled with the generated sample ID
+*In addition to your samples you will need to provide an aliquot of the primer
+**Volume is 10ul/sample you submit
+**Should be labeled as the range of sample IDs generated and P1 (example: xxxx-xxxxP1), if you have more than one primer write xxxx-xxxxP2 and so on
+*Go to https://seqcore.brcf.med.umich.edu/sample-dropoff-locations for locations and drop off times
+*They will send you an email when the project has been completed. You can download the .ab1 files here: https://client-seqcore.brcf.med.umich.edu/ftpdata.cgi