Chromatin Immunoprecipitation: Difference between revisions
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2. Add formaldehyde to a final concentration of 1% directly to the media of adherent cells growing on tissue culture plates, swirl gently, and | 2. Add formaldehyde to a final concentration of 1% directly to the media of adherent cells growing on tissue culture plates, swirl gently, and | ||
incubate at room temperature for 10 minutes | incubate at room temperature for 10 minutes. | ||
3. Stop the cross-linking reaction by adding glycine to a final concentration of 0.125M and swirl gently to mix. | 3. Stop the cross-linking reaction by adding glycine to a final concentration of 0.125M (If using 10cm dishes add 0.5mL of the 2.5M glycine stock solution) and swirl gently to mix. | ||
4. Remove media from plates and wash cells with equal volume cold (4°C) 1X PBS. | 4. Remove media from plates and wash cells with equal volume cold (4°C) 1X PBS. | ||