Changes

QPCR

558 bytes added, 20:20, 1 July 2015

→‎Plate Preparation

#You can prepare the plate ~3h beforehand, keeping it at 4C

#Immediately before the run spin the plate briefly in the swinging bucket centrifuge.

===Run Protocol===

#Open LightCycler software.

#Click new experiment.

#Choose SYBR Green option from drop down and make sure that the correct block is in place (it will say 96 or 384).

#Click apply template.

#Go to templates>run templates> bridges SYBR Green 384.

#Load plate and click 'start run'.

#This protocol is set to do the following:

#Activate- 1 cycle @95 degrees for 10s.

#Amplify- 45 cycles @95, 60 and 73 degrees for 15s, 15s and 10s, respectively.

#Melt- 1 cycle @95, 40, 65, 95 and 40 degrees for 1m, 1m, 1s, continuous and 10s, respectively.

==Calculations==

162

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