Changes

*cDNA: see [[First_Strand_cDNA_Synthesis_(AB_Kit)|First Strand cDNA Synthesis (AB Kit)]] for details*SyberGreen PCR Master Mix Applied Biosystems(ThermoFisher Catalog # 4367659; [https://www.thermofisher.com/order/catalog/product/4367659 Vendor Link])*384 well qPCR plate(ThermoFisher Catalog # 4309849) and covers (Catalog # 4360954)*Primers (Dilute to 0.4 uM mixture of fwd and rev. From 100 uM stocks this is 4uL Forward Primer, 4 uL Reverse Primer and 992 uL Water). Generate This primer mix can be stored at -20 in a Working Primers box. Design primers according to [[Primer Design for qPCR]]