Assessing isoproterenol-stimulated whole-body lipolysis in vivo: Difference between revisions
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#Inject 10 mg/kg isoproterenol (prepared fresh in sterile PBS) via intraperitoneal injection. | #Inject 10 mg/kg isoproterenol (prepared fresh in sterile PBS) via intraperitoneal injection. | ||
#Wait 15 minutes. | #Wait 15 minutes. | ||
#Briefly anesthetize mice with isoflurane and collect blood via retro orbital bleed. Allow blood to clot over ice. | #Briefly anesthetize mice with isoflurane and collect blood via retro orbital bleed. Allow blood to clot over ice. ***Note: Take care when anesthetizing mice the second time. They will go under VERY quickly, so watch the mouse closely*** | ||
#Centrifuge blood samples and collect serum. | #Centrifuge blood samples and collect serum. | ||
#Assay serum for NEFA's and Glycerol/Triglycerides using Wako and Sigma assay kits, respectively. | #Assay serum for NEFA's and Glycerol/Triglycerides using Wako and Sigma assay kits, respectively. | ||