User contributions for Erinstephenson

9 May 2016

• 20:3920:39, 9 May 2016 diff hist +244 m Single fiber dissociation of intact rodent muscles No edit summary current
• 20:3420:34, 9 May 2016 diff hist +1,794 N Single fiber dissociation of intact rodent muscles This protocol is for isolating single muscle fibers from intact mouse skeletal muscles. It is optimized for use with the Flexor Digitorum Brevis (superficial foot) muscle.

12 June 2015

• 15:3815:38, 12 June 2015 diff hist +27 Assessing isoproterenol-stimulated whole-body lipolysis in vivo No edit summary

10 March 2015

• 15:4715:47, 10 March 2015 diff hist +45 Assessing isoproterenol-stimulated whole-body lipolysis in vivo →NEFA determination from serum (use Wako Diagnostics NEFA-HR(2) Microtiter Kit)
• 15:4415:44, 10 March 2015 diff hist −4 Assessing isoproterenol-stimulated whole-body lipolysis in vivo →NEFA determination from serum (use Wako Diagnostics NEFA-HR(2) Microtiter Kit)
• 15:4415:44, 10 March 2015 diff hist +449 Assessing isoproterenol-stimulated whole-body lipolysis in vivo →NEFA determination from serum (use Wako Diagnostics NEFA-HR(2) Microtiter Kit)
• 15:2115:21, 10 March 2015 diff hist +124 m Assessing isoproterenol-stimulated whole-body lipolysis in vivo →Experimental protocol

19 February 2015

• 20:4920:49, 19 February 2015 diff hist +16 Extraction of DNA from TRIZOL preparations No edit summary
• 20:4820:48, 19 February 2015 diff hist +93 Extraction of DNA from TRIZOL preparations →Extraction steps
• 20:4620:46, 19 February 2015 diff hist +2,524 N Extraction of DNA from TRIZOL preparations This protocol is for extracting DNA from TRIZOL preparations after the RNA-containing upper phase has been completely removed

13 October 2014

• 22:1822:18, 13 October 2014 diff hist +1,289 N H&E Staining Created page with "== Rehydrate tissue sections == #After cutting sections of tissue onto glass slides, de-pariffinize the tissue sections in xylene for 3 min. Repeat for a total of 3x 3 minute ..."

22 September 2014

• 21:4421:44, 22 September 2014 diff hist −4,100 Paraffin Embedding of Tissue Samples No edit summary

14 August 2014

• 21:5221:52, 14 August 2014 diff hist +1,090 N How to design primer pairs for determining the presence of CrispR-cas induced mutations Created page with "#Go to http://www.ncbi.nlm.nih.gov/nuccore/ and find the sequence of interest. #Scroll down to the sequence and highlight/copy the nucleotides corresponding with your CrispR ..." current

24 July 2014

• 18:1818:18, 24 July 2014 diff hist −2 m Assessing isoproterenol-stimulated whole-body lipolysis in vivo No edit summary
• 18:1718:17, 24 July 2014 diff hist −52 m Assessing isoproterenol-stimulated whole-body lipolysis in vivo No edit summary
• 18:1518:15, 24 July 2014 diff hist +70 m Assessing isoproterenol-stimulated whole-body lipolysis in vivo No edit summary
• 18:1318:13, 24 July 2014 diff hist −49 Assessing isoproterenol-stimulated whole-body lipolysis in vivo No edit summary
• 18:1218:12, 24 July 2014 diff hist +498 Assessing isoproterenol-stimulated whole-body lipolysis in vivo No edit summary
• 17:2817:28, 24 July 2014 diff hist +2,381 Assessing isoproterenol-stimulated whole-body lipolysis in vivo No edit summary
• 14:4814:48, 24 July 2014 diff hist +1,091 N Assessing isoproterenol-stimulated whole-body lipolysis in vivo Created page with "== Assessing Isoproterenol-Stimulated Whole-Body Lipolysis ''in vivo'' == ##Note: Mice do not need to be in a fasted state prior to this test. #Briefly anesthetize mice with ..."

22 July 2014

• 18:4418:44, 22 July 2014 diff hist +509 Paraffin Embedding of Tissue Samples No edit summary
• 18:2418:24, 22 July 2014 diff hist +1,707 Paraffin Embedding of Tissue Samples No edit summary

17 February 2014

• 17:3517:35, 17 February 2014 diff hist +80 m Rapamycin Injections No edit summary
• 17:2417:24, 17 February 2014 diff hist 0 m Rapamycin Injections No edit summary